abstract：:We have developed an in vivo mouse model [the green fluorescent protein (GFP) / carbon tetrachloride (CCl(4)) model] and reported that infused GFP-positive bone marrow cells administered via a tail vein efficiently repopulated cirrhotic liver tissue under conditions of persistent liver damage induced by CCl(4). Moreov...

journal_title：Journal of pharmacological sciences

authors： Takami T,Terai S,Sakaida I

更新日期：2011-01-01 00:00:00

abstract：:Peripheral-type benzodiazepine receptor (PBR) and central-type benzodiazepine receptor (CBR) in salivary gland play a role in the inhibitory regulation of salivary secretion in rodents. Diazepam-binding inhibitor (DBI), an endogenous ligand for PBR, produces neurosteroids, which modulate CBR activity. In this study, w...

journal_title：Journal of pharmacological sciences

authors： Tsukagoshi E,Kawaguchi M,Shinomiya T,Yoshikawa M,Kawano T,Okubo M,Sawaki K

更新日期：2011-01-01 00:00:00

abstract：:We aimed to examine the rate of thrombotic events after discontinuation of one year clopidogrel therapy in patients with implanted coronary stent, and to determine platelet aggregability by multiple electrode analyzer after cessation of clopidogrel. This prospective, multicenter study enrolled 200 patients subjected t...

journal_title：Journal of pharmacological sciences

authors： Djukanovic N,Todorovic Z,Obradovic S,Zamaklar-Trifunovic D,Njegomirovic S,Milic NM,Prostran M,Ostojic M

更新日期：2011-01-01 00:00:00

abstract：:The purpose of this study is to identify transient receptor potential canonical (TRPC) channels responsible for receptor-operated Ca(2+) entry (ROCE) triggered by activation of endothelin type A receptor (ET(A)R) and to clarify the importance of calmodulin (CaM) / inositol 1,4,5-trisphosphate (IP(3)) receptor binding ...

journal_title：Journal of pharmacological sciences

authors： Horinouchi T,Terada K,Higa T,Aoyagi H,Nishiya T,Suzuki H,Miwa S

更新日期：2011-01-01 00:00:00

abstract：:Arachidonic acid (ARA) and docosahexaenoic acid (DHA) are major constituents of cell membranes and play important roles in preserving physiological and psychological function. Recently, data from several studies have indicated that impairments in long-term potentiation (LTP), the process underlying plasticity in synap...

journal_title：Journal of pharmacological sciences

authors： Kiso Y

更新日期：2011-01-01 00:00:00

abstract：:Cytochrome P450 (CYP)-mediated drug interactions caused by Kampo medicine have not been investigated sufficiently. The current study was conducted to reveal the effect of anchusan, a commonly used Kampo formula for gastrointestinal disease, on CYP3A-mediated drug metabolism in rats. The pharmacokinetics of midazolam (...

journal_title：Journal of pharmacological sciences

authors： Saito Y,Nishimura Y,Kurata N,Iwase M,Aoki K,Yasuhara H

更新日期：2011-01-01 00:00:00

abstract：:Vascular endothelial cells (VECs) secrete tissue plasminogen activator (tPA) in an active form and thus its facilitated secretion directly enhances fibrinolytic activity. We have recently demonstrated its unique secretory dynamics in GFP-tagged tPA expressing VECs using total internal reflection fluorescence microscop...

journal_title：Journal of pharmacological sciences

authors： Suzuki Y,Urano T

更新日期：2011-01-01 00:00:00

abstract：:Cigarette smoking is one of the factors causing accumulation of vascular smooth muscle cells (VSMCs) in atherosclerotic plaques. Changes in cell migration toward platelet-derived growth factor BB were investigated using a Boyden chamber after 48-h preincubation of GBaSM-4 VSMCs with nicotine or nicotine-free cigarette...

journal_title：Journal of pharmacological sciences

authors： Yoshiyama S,Horinouchi T,Miwa S,Wang HH,Kohama K,Nakamura A

更新日期：2011-01-01 00:00:00

abstract：:Atherosclerotic lesions were observed in male and ovariectomized female Microminipig (MMP) fed a high fat and cholesterol diet with sodium cholate (HFCD/SC) for 3 months. HFCD/SC induced hypercholesterolemia accompanied by an increase in serum total cholesterol (T-Cho), low-density lipoprotein cholesterol (LDL-C), hig...

journal_title：Journal of pharmacological sciences

authors： Kawaguchi H,Miyoshi N,Miura N,Fujiki M,Horiuchi M,Izumi Y,Miyajima H,Nagata R,Misumi K,Takeuchi T,Tanimoto A,Yoshida H

更新日期：2011-01-01 00:00:00

abstract：:Sneezing and persistent itching of the nasal mucosa are distressing symptoms of allergic rhinitis (AR). Recent studies have revealed that hyperinnervation of sensory neurons in the nasal turbinate is one of the underlying causes of sneezing and itching. Since Semaphorin-3A (Sema3A) has been previously shown to restric...

journal_title：Journal of pharmacological sciences

authors： Sawaki H,Nakamura F,Aihara M,Nagashima Y,Komori-Yamaguchi J,Yamashita N,Nakazawa M,Goshima Y,Ikezawa Z

更新日期：2011-01-01 00:00:00

abstract：:Mosapride citrate (mosapride), a prokinetic agent with 5-HT(4)-receptor agonistic activity, is known to enhance gastric emptying and alleviate symptoms in patients with functional dyspepsia (FD). As hyperalgesia and delayed gastric emptying play an important role in the pathogenesis of FD, we used in this study balloo...

journal_title：Journal of pharmacological sciences

authors： Seto Y,Yoshida N,Kaneko H

更新日期：2011-01-01 00:00:00

abstract：:Protective effect of valsartan (Val), an angiotensin II (AII)-receptor blocker (ARB), against organ damage is reported to depend on the dosing time in hypertensive patients. Dosing-time-dependent effect of Val on survival of stroke-prone spontaneously hypertensive rats (SHRSP) under a 12-h lighting cycle was examined....

journal_title：Journal of pharmacological sciences

authors： Liu Y,Ushijima K,Ohmori M,Takada M,Tateishi M,Ando H,Fujimura A

更新日期：2011-01-01 00:00:00

abstract：:Stiffness and cytokine in blood levels show 24-h rhythms in rheumatoid arthritis (RA) patients. We previously revealed that higher therapeutic effects were obtained in RA patients and RA model animals when the dosing time of methotrexate was chosen according to the 24-h rhythms to cytokine. In this study, we examined ...

journal_title：Journal of pharmacological sciences

authors： Obayashi K,Tomonari M,Yoshimatsu H,Fukuyama R,Ieiri I,Higuchi S,To H

更新日期：2011-01-01 00:00:00

abstract：:The effects of AS1892802, a selective Rho-associated coiled coil kinase (ROCK) inhibitor, on knee cartilage damage and pain behavior were examined in a rat model of osteoarthritis (OA). Monoiodoacetate (MIA) was intraarticularly injected into the right knee joints of rats. ROCK I and II mRNA levels increased in knee j...

journal_title：Journal of pharmacological sciences

authors： Takeshita N,Yoshimi E,Hatori C,Kumakura F,Seki N,Shimizu Y

更新日期：2011-01-01 00:00:00

abstract：:We investigated the inhibitory effects of β₁- or β₂-adrenoceptor (AR) antagonists on salivary amylase secretion produced by various emetic agents, such as cisplatin, apomorphine, and lithium chloride (LiCl), or the non-emetic agent β(½)-AR agonist isoprenaline in rats. We also determined the inhibitory effect of metoc...

journal_title：Journal of pharmacological sciences

authors： Fukui H,Suyama Y,Iwachido T,Miwa E

更新日期：2011-01-01 00:00:00

abstract：:G protein-coupled receptors, in particular, Ca(2+)-mobilizing G(q)-coupled receptors have been reported to be targets for anesthetics. Opioids are commonly used analgesics in clinical practice, but the effects of anesthetics on the opioid mu-receptors (muOR) have not been systematically examined. We report here an ele...

journal_title：Journal of pharmacological sciences

authors： Minami K,Sudo Y,Shiraishi S,Seo M,Uezono Y

更新日期：2010-01-01 00:00:00

abstract：:We have reported that the differentiation-inducing factors (DIFs) DIF-1 and DIF-3, morphogens secreted from Dictyostelium discoideum, inhibit proliferation of several cancer cells via suppression of the Wnt/beta-catenin signaling pathway. However, the target molecules of DIFs involved in the anti-proliferative effects...

journal_title：Journal of pharmacological sciences

authors： Matsuda T,Takahashi-Yanaga F,Yoshihara T,Maenaka K,Watanabe Y,Miwa Y,Morimoto S,Kubohara Y,Hirata M,Sasaguri T

更新日期：2010-01-01 00:00:00

abstract：:The critical role played by the renin-angiotensin-aldosterone system (RAAS) in the regulation of blood pressure and body fluid homeostasis has been well recognized. Angiotensin (Ang) II and aldosterone are the most powerful biologically active products of the RAAS, although there are also other bioactive Ang peptides ...

journal_title：Journal of pharmacological sciences

authors： Nishiyama A,Kim-Mitsuyama S

更新日期：2010-01-01 00:00:00

abstract：:2-Aminophenoxazine-3-one (Phx-3) induced cellular apoptosis in mouse melanoma B16 cells as detected by DNA laddering and upregulated Fas expression in the cells in vitro. Next, the anti-metastatic effects of Phx-3 were investigated in C56BL/6 mice. When B16 melanoma cells were injected into the tail veins of mice, sig...

journal_title：Journal of pharmacological sciences

authors： Hongo T,Miyano-Kurosaki N,Kurosaki K,Hata A,Harigae S,Tomoda A

更新日期：2010-01-01 00:00:00

abstract：:The L-type Ca(2+) channel (Ca(V)1.2) shows clear Ca(2+)-dependent facilitation and inactivation. Here we have examined the effects of calmodulin (CaM) and Ca(2+) on Ca(2+) channel in guinea-pig ventricular myocytes in the inside-out patch mode, where rundown of the channels was controlled. At a free [Ca(2+)] of 0.1 mi...

journal_title：Journal of pharmacological sciences

authors： Han DY,Minobe E,Wang WY,Guo F,Xu JJ,Hao LY,Kameyama M

更新日期：2010-01-01 00:00:00

abstract：:We investigated the roles of β- and α(2)-adrenoceptors within the central nucleus of the amygdala (CeA) in the negative affective and sensory components of visceral pain in rats. We observed a dose-dependent reduction of intraperitoneal acetic acid-induced conditioned place aversion by bilateral injections of timolol,...

journal_title：Journal of pharmacological sciences

authors： Deyama S,Takishita A,Tanimoto S,Ide S,Nakagawa T,Satoh M,Minami M

更新日期：2010-01-01 00:00:00

abstract：:RhoA upregulation has been suggested in bronchial smooth muscles (BSMs) of asthmatic rats. Here, we cloned/characterized the 5'-promoter region of the rat rhoA. A transcription-initiation site was identified at 66-bp upstream of the reference sequence, GenBank-BC061732. Luciferase assay using interleukin-13 (IL-13)-st...

journal_title：Journal of pharmacological sciences

authors： Chiba Y,Goto K,Matsusue K,Kimura S,Misawa M

更新日期：2010-01-01 00:00:00

abstract：:We investigated the effects of treatment with trimethyltin (TMT) on the expression of glutathione-related enzymes in mouse hippocampus. TMT promoted the expression of glutathione S-transferase (GST) Ya/Yc mRNA, and GSTA2 protein, but not that of glutamate-cysteine ligase catalytic subunit mRNA, 1 day after injection. ...

journal_title：Journal of pharmacological sciences

authors： Nagashima R,Sano S,Huong NQ,Shiba T,Ogita K

更新日期：2010-01-01 00:00:00

abstract：:Epigenetics is a mechanism that regulates gene expression not depending on the underlying DNA sequence, but on the chemical modifications of DNA and histone proteins. Defects in the factors involved in epigenetic regulation cause congenital neurodevelopmental diseases, and thus, epigenetic regulation is essential for ...

journal_title：Journal of pharmacological sciences

authors： Kubota T,Miyake K,Hirasawa T,Nagai K,Koide T

更新日期：2010-01-01 00:00:00

abstract：:Although the opioid system is known to modulate depression-like behaviors, its role in the effects of antidepressants is not yet clear. We investigated the role of μ-opioid receptors (MOPs) in the effects of venlafaxine, a serotonin and norepinephrine reuptake inhibitor, in the forced swim test using MOP-knockout (KO)...

journal_title：Journal of pharmacological sciences

authors： Ide S,Fujiwara S,Fujiwara M,Sora I,Ikeda K,Minami M,Uhl GR,Ishihara K

更新日期：2010-01-01 00:00:00

abstract：:Forsythiae Fructus is known to have diuretic, anti-bacterial, and anti-inflammatory activities. This study examined the hepatoprotective effects of pinoresinol, a lignan isolated from Forsythiae Fructus, against carbon tetrachloride (CCl(4))-induced liver injury. Mice were treated intraperitoneally with vehicle or pin...

journal_title：Journal of pharmacological sciences

authors： Kim HY,Kim JK,Choi JH,Jung JY,Oh WY,Kim DC,Lee HS,Kim YS,Kang SS,Lee SH,Lee SM

更新日期：2010-01-01 00:00:00

abstract：:Aristolochic acids (AAs), contained in Chinese herbal preparations, have been considered to induce nephropathy. In order to elucidate the molecular mechanisms of AA-induced nephrotoxicity, we have elucidated the interaction of human organic anion transporters (hOATs) with AAs using their stable cell lines. AA-I and AA...

journal_title：Journal of pharmacological sciences

authors： Babu E,Takeda M,Nishida R,Noshiro-Kofuji R,Yoshida M,Ueda S,Fukutomi T,Anzai N,Endou H

更新日期：2010-01-01 00:00:00

abstract：:MicroRNAs (miRNAs) are a group of small noncoding RNAs that regulate translational repression of multiple target mRNAs. The miRNAs in a whole cell regulate greater than 30% of all protein-coding genes. The vast majority of presently identified miRNAs are expressed in the brain in a spatially and temporally controlled ...

journal_title：Journal of pharmacological sciences

authors： Satoh J

更新日期：2010-01-01 00:00:00

abstract：:This study examines the influence of receptor expression level on signaling pathways activated via endothelin type A receptor (ET(A)R) expressed in Chinese hamster ovary cells at 32,100 (ET(A)R-high-CHO) and 893 (ET(A)R-low-CHO) fmolmg protein(-1). Endothelin-1 (ET-1) elicited a sustained increase in intracellular Ca(...

journal_title：Journal of pharmacological sciences

authors： Horinouchi T,Asano H,Higa T,Nishimoto A,Nishiya T,Muramatsu I,Miwa S

更新日期：2009-12-01 00:00:00

abstract：:Cisplatin causes chronic interstitial disease with fibrosis, but the development mechanism of interstitial fibrosis is not yet understood. We examined the effect of an antioxidant, N,N'-diphenyl-1,4-phenylenediamine (DPPD), on development of interstitial fibrosis induced by cisplatin. Cisplatin increased blood urea ni...

journal_title：Journal of pharmacological sciences

authors： Kawai Y,Satoh T,Hibi D,Ohno Y,Kohda Y,Miura K,Gemba M

更新日期：2009-12-01 00:00:00

abstract：:The pharmacological effects of rivoglitazone, a novel thiazolidinedione-derivative peroxisome proliferator-activated receptor (PPAR)-gamma agonist, were characterized in vitro and in vivo. Rivoglitazone activated human PPARgamma more potently compared with rosiglitazone and pioglitazone and had little effect on PPARal...

journal_title：Journal of pharmacological sciences

authors： Kanda S,Nakashima R,Takahashi K,Tanaka J,Ogawa J,Ogata T,Yachi M,Araki K,Ohsumi J

更新日期：2009-10-01 00:00:00

abstract：:We studied the effects of antipsychotics and a hypnotic on sleep disturbance in schizophrenia using an animal model of the disease. Electrodes for the electroencephalogram (EEG) and electromyogram (EMG) were chronically implanted into the cortex and the dorsal neck muscle of rats. EEG and EMG were recorded with an ele...

journal_title：Journal of pharmacological sciences

authors： Ishida T,Obara Y,Kamei C

更新日期：2009-09-01 00:00:00

abstract：:Aspirin (acetylsalicylic acid) is a well-known nonsteroidal anti-inflammatory drug that can potentiate some acute allergies and causes adverse immunological reactions collectively referred to as aspirin intolerance, a disorder that induces urticaria, asthma, and anaphylaxis in response to oral administration of the dr...

journal_title：Journal of pharmacological sciences

authors： Suzuki Y,Ra C

更新日期：2009-07-01 00:00:00

abstract：:Genetically epilepsy-prone rats (GEPR-9s) were derived from Sprague-Dawley rats (SD). The number of kainate-induced wet dog shake behavior (WDS) responses was found to decrease significantly in GEPR-9s compared to SD. WDS responses were potentiated by 5-hydroxytryptophan or 2,5-dimethoxy-4-iodoamphetamine and antagoni...

journal_title：Journal of pharmacological sciences

authors： Shin EJ,Jeong JH,Chung YH,Kim TW,Shin CY,Kim WK,Ko KH,Kim HC

更新日期：2009-07-01 00:00:00

abstract：:It has been shown that enhancement of vasoconstrictor prostanoids plays an important role in the development of cardiovascular diseases. The aim of the present study was to examine the effects of pyrrolidine dithiocarbamate (PDTC), a low-molecular-weight thiol antioxidant and a potent inhibitor of nuclear factor-kappa...

journal_title：Journal of pharmacological sciences

authors： Matsumoto T,Ishida K,Kobayashi T,Kamata K

更新日期：2009-07-01 00:00:00

abstract：:The involvement of Ca2+ entry via the Na+/Ca2+ exchanger (NCX) in myogenic constriction of rat posterior cerebral arteries was investigated. RT-PCR identified mRNA for NCX1, 2, and 3 in the arteries. Na+ removal increased [Ca2+]i, which was reduced by the NCX inhibitor SEA0400. SEA0400 inhibited the development, but n...

journal_title：Journal of pharmacological sciences

authors： Kashihara T,Nakayama K,Matsuda T,Baba A,Ishikawa T

更新日期：2009-06-01 00:00:00

abstract：:Mood disorders are not merely attributed to the functional defect of neurotransmission, but also are due to the structural impairment of neuroplasticity. Chronic stress decreases neurotrophin levels, precipitating or exacerbating depression; conversely, antidepressants increase expression of various neurotrophins (e.g...

journal_title：Journal of pharmacological sciences

authors： Wada A

更新日期：2009-05-01 00:00:00

abstract：:Ca(2+) release from intracellular store sites via the ryanodine receptor (RyR) and hormonal regulation by flutamide, an androgen-receptor (AR) antagonist, on it were examined in vas deferens (VD) smooth muscle cells (SMCs). VD and VDSMCs were obtained from two groups of male rats that were treated p.o. with 100 mg/kg ...

journal_title：Journal of pharmacological sciences

authors： Ohno A,Ohya S,Yamamura H,Imaizumi Y

更新日期：2009-05-01 00:00:00

abstract：:To elucidate the mechanism of action of prostaglandin I(2) (PGI(2)) and carbaprostacyclin, we studied their effect on DNA synthesis and proliferation in primary cultures of adult rat hepatocytes. Hepatocyte parenchymal cells, maintained in a serum-free, defined medium, synthesized DNA and proliferated in the presence ...

journal_title：Journal of pharmacological sciences

authors： Kimura M,Okamoto H,Natsume H,Ogihara M

更新日期：2009-04-01 00:00:00

abstract：:Our previous study showed that organobismuth compounds induce apoptosis in human promyelocytic leukemia cells, although solid tumor cell lines were relatively resistant. Herein, we investigated the primary cellular target of these compounds in HeLa cells. One organobismuth compound, bi-chlorodibenzo[c,f][1,5]thiabismo...

journal_title：Journal of pharmacological sciences

authors： Iuchi K,Akagi K,Yagura T

更新日期：2009-04-01 00:00:00